Research - Does Chocolate Make You Clever

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  Research Chocolate vs cognitive Desideri G, Kwik-Uribe C, Grossi D et al. Benefits in cognitive function, blood pressure, and insulin resistance through cocoa flavours consumption in elderly subjects with mild cognitive impairment: the cocoa cognitive , and aging (Cocoa) study. Hypertension. 2012;60 (3): 794  –  801 Consume a daily cocoa drink with differing flavanol content, tested on 90 individuals with mild cognitive impairment  –  8 weeks. Tested using drinks with 990mg, 520mg and 45mg of flavonoids. Cognitive function assessed by:    Mini mental state examinations    Trial making tests A and B    Verbal fluency tests Key findings: after 8 weeks, participants consuming high and intermediate flavanol cocoa products and significantly better in the tests. Benefits significant at 500mg/day Earlier studies have reported cognitive benefits from flavanol sourced from other foods. (Concord grape juice, 2010) May 2013  –   similar study on chocolate flavanol’s effects on cognition and mood –  self-rated calmness and contentedness, but cognition was unaffected by chocolate in this study. Flavanols that occur naturally in cocoa beans are easily destroyed during chocolate manufacturing. Dark chocolate contains more flavanols than milk or white chocolate. Among dark chocolate, there was almost 7-fold variation in content. Low flavanols experimental group consumed commercial chocolate drinks. High-falvoured content: cocoa pro produced by Mars Inc. Presentation: Cocoa Polyphenols. Pennington Biomedical Research Center Division of Education. Heli Roy, Shanna Lundy, Phillip Brantley, PBRC 2005 Cocoa and chocolate products have the highest concentration of flavonoids among commonly consumed foods. Only 10% of cocoa powder is flavonoids Switzerland  –  highest chocolate consumption  –  estimated 9.9kg/yr/person Flavanoids in cocoa/chocolate, catechin and epicatechin, exist in long polymers - up to 10 catechin/epicatechin units linked (other flavonoid rich foods contain 2/3 in each chain). Known to have hydrogen-donating properties and metal-chelating antioxidant properties  Roasting and alkali treatment to cocoa beans reduce flavanol content, alongside fermentation of the beans  –  As much as 90% of the flavonoids can be lost during processing 7% to 35% cocoa in milk chocolate 30% to 80% cocoa in dark chocolate 0% cocoa in white chocolate Factsheet: CVS Health Resouces. 8/1/2011, EBSCO CAM Review Board. Pg 2.  White chocolate contains little to no flavonoids. Commercial dark chocolate can contain up to 500-2000mg of flavanols per 100g of chocolate Pase MP, Scholey AB, Piping as A et al. 2013 May; 27(5): 451-8 J Psychopharmacol. Cocoa polyphenols enhance positive mood states but not cognitive performance: a randomised placebo controlled trial. Centre for Human Psychopharmacology, Swinburne University, Melbourne, Australia. Healthy middle aged participants Drink chocolate drinks with 500mg, 250mg and 0mg of flavanols  –  drink for 30 days Cognition measured using the Cognitive Drug Research system. 72 participants Cognition was unaffected by treatment at all time points  –  immediately, several hours after, and 1 month later His Test? Original data? The New England Journal of Medicine, Franz H. Messerli, Chocolate Consumption, Cognitive Function and Nobel Laureates. 2012 Massachusetts Medical Society, page 1-3 Testing for correlation between a country’s level of chocolate consumption and its population’s cognitive function  –   measured population’s cognitive function through the number of Nobel Laureates per capita. No. of Nobel laureates for every 10 million persons in a given country. Data from Chocosuisse, Theobroma-cacao and Caobisco. Data available from 2011 for Switzerland, 2010 for 15 countries, 2004 for 5 countries and 2002 for China R= 0.791  P < 0.0001 Implies a significant linear correlation Excluded Sweden: r = 0.862 Slope: 0.4kg chocolate per capita per year to increase the number of Nobel laureates in a given country by 1. Minimally effective chocolate dose: 2kg per year. No apparent ceiling on the no. of Nobel laureates at the highest chocolate dose level of 11kg per year. Graph: Sweden = anomaly. Very high no. of Nobel laureates, not so much chocolate consumption. 23 countries tested According to correlation, given 6.4kg/yr/capita, Sweden should have had 14 Nobel laureates but it has 32. Ruled Sweden as an anomaly because observed number > 2x expected number. Present data based on country averages Not known about the specific chocolate intake of the Nobel laureates Dr Messerli reports regular daily chocolate consumption, mostly but not exclusively, in the form of Lindt’s dark varieties   Malawi Med J. Sept 2012; 24(3): 69-71. A guide to appropriate use of correlation coefficient in medical research MM Mukaka 2013 Lund Research Ltd. Laerd Statistics, Pearson Product-Moment Correlation  The Pearson product-moment correlation does not take into consideration whether a variable has been classified as a dependant or independent variable. Assumptions:    Variables must be either interval or ratio measurements    Variables must be normally distributed    Linear relationship     Outliers are removed    Homoscedasticity (variances along line of best fit remain similar as you move along the line) At Work, Issue 40, Spring 2005: Institute for Work and Health, Toronto. What researchers mean by…statistical significance. ‘P’ to describe the probability of observing such a large difference purely by chance in 2 groups of exactly the same people. So 0.0001 means 0.01% chance that not correlated Below 0.005  –  considered highly statistically significant Pg 2: Topic #7: P-Value. Cornell edu.      P-value is not the probability that the null hypothesis is true. Comparison of Bayesian and classical approaches shows that a p-value can be very close to 0 while the posterior probability of the null is very close to unity (Jeffreys-Lindley paradox)    Calculation of p-value is based on assumption that finding is the product of chance alone    Prosecutor’s fallacy : not the probability of falsely rejecting the null hypothesis    Significance level of the test is not determined by the p-value
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